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9. | | FINGER, F. L.; ENDRES, L.; MOSQUIM, P. R.; PUIATTI, M. Physiological changes during postharvest senescence of broccoli. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 34, n. 9, p. 1565-69, set. 1999 Título em português: Alterações fisiológicas da senescência pós-colheita de brócolis Biblioteca(s): Embrapa Hortaliças; Embrapa Unidades Centrais. |
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12. | | VIEIRA, M. C.; CASALI, V. W. D.; CARDOSO, A. A.; MOSQUIM, P. R. Competicao de quatro clones de mandioquinha-salsa, em Dourados-MS. Horticultura Brasileira, Brasilia, v.13, n.1, p.121, maio 1995. Resumo. Biblioteca(s): Embrapa Hortaliças. |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
05/11/2020 |
Data da última atualização: |
05/11/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
SOARES, S. L.; ANCIUTIA, A. N.; DIAS, L.; CORCINI, C. D.; VARELA JÚNIOR, A. S.; KOMNINOU, E. R.; TEBALDI, M. L.; MARQUES, M. G.; FONSECA, F. M. da; LUCIA JUNIOR, T. |
Afiliação: |
SARA L. SOARES, UFPel; ANDREIA N. ANCIUTI, UFPel; LUCIA DIAS, UFPel; CARINE DAHL CORCINI, UFPel; ANTÔNIO S. VARELA JÚNIOR, FURG; ELIZA R. KOMNINOU, UFPel; MARLI LUIZA TEBALDI, UNIFEI; MARIANA GROKE MARQUES, CNPSA; FRANCISCO NOE DA FONSECA, CNPSA; THOMAZ LUCIA JUNIOR, UFPel. |
Título: |
Safety assessment of poly (N-vinylcaprolactam) as a potential drug carrier in extenders for boar sperm cryopreservation. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Toxicology in Vitro, v. 65, n. 104766, 2020. |
DOI: |
https://doi.org/10.1016/j.tiv.2020.104766 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Polymers may be used to deliver compounds in freezing extenders to minimize injuries in spermatozoa during cryopreservation, although their activity and toxicity for boar sperm are unknown. This study investigated the effects of the polymer (N-vinylcaprolactam) (PNVCL), when included in extenders for boar sperm cryopreservation. In Experiment 1, sperm was exposed to PNVCL at: 0 (control); 39.1; 78.1; 156.3; and 312.5 μg/mL. Spermatozoa structure, kinetics and biochemical functions were unaltered in contact with PNVCL at 38 °C (P > .05) but declined with prolonged exposure (10, 60 and 120 min) in all treatments (P > .05). In Experiment 2, after inclusion of PNVCL in the freezing extender at the same concentrations, post-thawing sperm quality did not differ compared to the control (P > .05). Lipid peroxidation and the production of reactive oxygen species were the only parameters of sperm quality that were unaffected in both experiments, even after contact with PNVCL for 120 min (P > .05). As no negative effects were observed in post-thawing boar sperm quality, PNVCL did not incur in cytotoxicity and may be a potential carrier for antioxidants in freezing extenders. |
Thesagro: |
Criopreservação; Espermatozóide; Javali; Reprodução Animal. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02092naa a2200289 a 4500 001 2126319 005 2020-11-05 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.tiv.2020.104766$2DOI 100 1 $aSOARES, S. L. 245 $aSafety assessment of poly (N-vinylcaprolactam) as a potential drug carrier in extenders for boar sperm cryopreservation.$h[electronic resource] 260 $c2020 520 $aAbstract: Polymers may be used to deliver compounds in freezing extenders to minimize injuries in spermatozoa during cryopreservation, although their activity and toxicity for boar sperm are unknown. This study investigated the effects of the polymer (N-vinylcaprolactam) (PNVCL), when included in extenders for boar sperm cryopreservation. In Experiment 1, sperm was exposed to PNVCL at: 0 (control); 39.1; 78.1; 156.3; and 312.5 μg/mL. Spermatozoa structure, kinetics and biochemical functions were unaltered in contact with PNVCL at 38 °C (P > .05) but declined with prolonged exposure (10, 60 and 120 min) in all treatments (P > .05). In Experiment 2, after inclusion of PNVCL in the freezing extender at the same concentrations, post-thawing sperm quality did not differ compared to the control (P > .05). Lipid peroxidation and the production of reactive oxygen species were the only parameters of sperm quality that were unaffected in both experiments, even after contact with PNVCL for 120 min (P > .05). As no negative effects were observed in post-thawing boar sperm quality, PNVCL did not incur in cytotoxicity and may be a potential carrier for antioxidants in freezing extenders. 650 $aCriopreservação 650 $aEspermatozóide 650 $aJavali 650 $aReprodução Animal 700 1 $aANCIUTIA, A. N. 700 1 $aDIAS, L. 700 1 $aCORCINI, C. D. 700 1 $aVARELA JÚNIOR, A. S. 700 1 $aKOMNINOU, E. R. 700 1 $aTEBALDI, M. L. 700 1 $aMARQUES, M. G. 700 1 $aFONSECA, F. M. da 700 1 $aLUCIA JUNIOR, T. 773 $tToxicology in Vitro$gv. 65, n. 104766, 2020.
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